Figure 1.

Mammary epithelial cells lacking CDH1 expression are preferentially sensitive to the cytotoxic effects of dasatinib. (A–D) Normalised MCF10a-WT and CDH1^−/− cell counts 48 h after treatment with serial dilutions of dasatinib (A), imatinib mesylate (B), ponatinib (C) and nilotinib (D). Wild-type, black bars, CDH1^−/− grey bars. Six fields per well at 4× magnification were captured using the Cytation 5 imager (Biotek). Nuclei were counted using Gen5 (Biotek) and normalised to the vehicle control for each cell line. (For all graphs, error bars = SEM; * p < 0.05, ** p < 0.01 and *** p <0.001; n ≥ 3 independent biological replicates; unpaired two-sided t-test).