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. 2022 Mar 25;12(7):832. doi: 10.3390/ani12070832

Figure 5.

Figure 5

Effects of MT supplementation on the distribution of mitochondria and cortical granules in DT-exposed porcine oocytes. (A) Representative images of mitochondria in control, DT-exposed and MT-supplemented groups. Oocytes in each group were stained for MitoTracker (red) and DNA (blue). The experiment was independently repeated three times with at least 35 oocytes per group. Scale bar: 50 µm. (B) The fluorescence intensity of mitochondrion signals was measured in control, DT-exposed and MT-supplemented groups. (C) Representative images of cortical granules localization in control, DT-exposed and MT-supplemented groups. The experiment was independently repeated three times with at least 40 oocytes per group. Scale bar: 50 µm. (D) The fluorescence intensity of cortical granules was measured in control, DT-exposed and MT-supplemented groups. All data are presented as mean ± S.E.M and different letters on the bars indicate significant differences (p < 0.05).