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. 2022 Mar 25;14(7):1673. doi: 10.3390/cancers14071673

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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SMYD3 knockdown functionally impairs MB cell proliferation. (A) Clonogenic assay using SMYD3 silenced and control D425 and D458 cells. (B) Colonies were quantified manually and graphically represented in the control and SMYD3 silenced cells. (C) Tunnel assay showing the apoptotic cells (green) and the nucleus (blue) in D425 and D458 cells. The fluorescence intensity was quantified using Image J. (D) Bar graph showing the percentage of apoptotic cells in shSMYD3 treated cells, compared with the control cells. (E) Annexin V assay using the RayBio^® Annexin V450 apoptosis kit in the control and shSMYD3 treated D425 and D458 cells. The statistical significance *** p < 0.001.