Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 25;14(7):1673. doi: 10.3390/cancers14071673

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

The effects of SMYD3 and cyclin D3 depletion on MB cell cytotoxicity. (A) MTT assay of D458 cells treated with cyclin D1/CDK4 or cyclin D3/CDK4 with or without SMYD3 knockout at 3 and 5 days. SMYD3 knockout (KO-SMYD3) CRISPR/Cas9 knockout all-in-one plasmids for specific deletion of three non-overlapping regions of SMYD3 (HCP260717-CG04-3-B) were used to treat D458 cells. (B,C) Bar graph depicting the percentage of control and siCCND3 treated D458 and MB002 cells in each phase of the cell cycle. (D) Western blot analysis of the nuclear extracts (NE) and cytoplasmic extracts (CE) obtained from the control and siCCND3 treated (48 h) D458 cells. Histone H1 and actin were used as the loading control for the NE and CE, respectively. Densitometry analysis for the cyclin D1 and D3 immunoblots of the NE and CE. The statistical significance * p < 0.05, ** p < 0.01, *** p < 0.001.