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. 2022 Mar 31;14(7):1782. doi: 10.3390/cancers14071782

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Investigation of hedgehog signaling pathway in thyroid cancer-derived cell lines. Three papillary thyroid cancer cell lines (BCPAP, K1, TPC-1) and three anaplastic thyroid cancer cell lines (BHT101, CAL62, SW1736) were subjected to western blot analysis. (A) Evaluation of the hedgehog signaling pathway. (B) Quantitative analysis of GLI1 expression in several thyroid cancer cell lines. ß-actin was used as an internal control. Mean ± standard deviation (SD) values from three independent experiments are presented. (C) Confirmation of NIS protein expression level in thyroid cancer-derived cell lines. ß-actin was used as an internal control. Raw data is presented in Figures S2–S4.