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. 2022 Apr 2;14(7):1814. doi: 10.3390/cancers14071814

Figure 7.

Figure 7

Plasma apelin is increased in cachectic cancer-bearing mice and it seems tumor-derived. ELISA was done on the plasma of mice with the cachectic tumors C26 (A), MCG101 (B) or LLC (C). Apelin was high in plasma of all three cancer types-bearing animals. Mann–Whitney test, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. PBS, C26, n = 4–5; PBS, MCG101, n = 10–14; PBS, LLC, n = 6–10. Proteins from 4T1 or C26 tumors excised from mice were analyzed in Western blot for apelin and GAPDH as loading control (D). Quantitation for apelin/GAPDH is shown (E). Unpaired t-test, ** p ≤ 0.01, n = 4–5. TA weight of non-cachectic and cachectic pancreatic cancer bearing-mice is shown (F), Unpaired t-Test, * p ≤ 0.05, n = 5–6. Q-PCR showed that the tumoral mRNA levels of apelin were higher in cachectic than in non-cachectic mice bearing pancreatic tumors and sacrificed, respectively, 31 vs. 26 days after tumor transplantation (G). Unpaired t-test, * p ≤ 0.05, n = 5–6.