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. 2022 Mar 22;11(7):1067. doi: 10.3390/cells11071067

Figure 6.

Figure 6

Psoralen promotes the initiation of autophagy. Sodium oleate-induced L02 cells were treated with psoralen (3.3 μM) alone or in combination with 3-MA (10 mM) for 24 h. (A) L02 cells were stained with the anti-LC3 antibody and Hoechst 33258 as detected by confocal microscopy (scale bar = 5 μm). (B) Quantification of LC3 puncta per cell. (C) The expression of p62, LC3I, and LC3II was measured by Western blotting. (D) L02 cells were stained with Nile red and Hoechst 33258 as detected by confocal microscopy (scale bar = 20 μm). (E) Quantification of Nile red fluorescence intensity per L02 cell. (F) The expression of FASN and CPT1a was measured by Western blotting. All values were expressed as the mean ± SD from three independent experiments. * p < 0.05, ** p < 0.01 vs. sodium oleate-induced control group. Abbreviations: 3-MA, 3-methyladenine; CPT1a, carnitine palmitoyltransferase 1a; Pso, psoralen; FASN, fatty acid synthase.