Figure 3.

PE infusion increases cancer cell proliferation. (A) Representative image of PyMT tumor sections of control and PE-infused mice stained with anti-Ki67 (proliferating cells; green) and DAPI (nuclei; blue). Scale bar: 50 µm. (B) Quantification of the number of proliferating cells in tumor section field (n = 4, at least 5 fields per each mouse). (C) PyMT cells were cultured for 48 h in the absence or presence of 10% FBS either with or without PE at 10 μg/mL, and mouse blood serum drawn from either control or PE-infused mice. Proliferation was measured using a Luminescent Cell Viability Assay using serum from at least three mice per group. Data are presented as mean ± SEM and were analyzed using either Student’s t-test (B) or one-way repeated-measures ANOVA, followed by Tukey posttests (C). * p < 0.05. *** p < 0.001.