TABLE 1.
Target | Primer | Oligonucleotide (5′→3′)a | Position | Tanb (°C) | Remarks |
---|---|---|---|---|---|
Subunit A topoisomerase IV | parC-f58 | TGA ATT TAC GGA AAA CGC CTA C | 58–79 | 60 | Sequencing |
parC-r591 | GCC ACT TCA CGC AGG TTA TG | 591–572 | 60 | Sequencing | |
marA operon, marR | maropR-f1394 | TTG CCT GGG CAA TAT TAT C | 1394–1411 | 45 | Sequencing |
maropR-r1950 | GTC CAA AAT GCT ATG AAT G | 1950–1928 | 45 | Sequencing | |
marop-f1140 | GCCAGGCCAAGAAATAAC | 1140–1157 | 50 | Sequencing | |
marop-r1556 | ATCCAGCGGAGACAGATAC | 1556–1538 | 50 | Sequencing | |
marA, marB | marAB-f1840 | GGC AAC ACT TGA GTA TTT GC | 1840–1859 | 50 | Sequencing |
marAB-r2704 | AGT AGG ACT GGC AAG TGC | 2704–2687 | 50 | Sequencing | |
marA | marA-f1893 | ATT AAG CTT ATG ACG ATG TCC AGA CG | 1893–1909 | 50 | HindIII site |
marA-r2281 | ATT TCT AGA ACT AGC TGT TGT AAT G | 2282–2267 | 50 | XbaI site | |
marR | marR-f1452 | AAT GAA TTC GTA CCA GCG ATC TGT TCA ATG | 1452–1472 | 50 | EcoRI site |
marR-r2229 | AAT CTG CAG TGG TCA TCC GGT ATT TAT GC | 2229–2210 | 50 | PstI site | |
soxS | soxS-f331 | ATA GAA TTC ACC AAT AAA ATT ACA GGC GG | 3331–3350 | 50 | EcoRI site |
soxS-r1319 | ATA CTC GAG ATG TCC CAT CAG AAA ATT ATT CAG | 1319–1297 | 50 | XhoI site | |
acrA | acrA-f31 | GCG GTC GTT CTG ATG CTC TC | 31–48 | 50 | Northern probe |
acrA-r330 | ACC TTT CGC ACT GTC GTA TG | 330–310 | 50 | Northern probe |
Restriction sites are italicized.
Tan, annealing temperature.