TABLE 1.

Primers used in this study

Target	Primer	Oligonucleotide (5′→3′)a	Position	Tanb (°C)	Remarks
Subunit A topoisomerase IV	parC-f58	TGA ATT TAC GGA AAA CGC CTA C	58–79	60	Sequencing
	parC-r591	GCC ACT TCA CGC AGG TTA TG	591–572	60	Sequencing
marA operon, marR	maropR-f1394	TTG CCT GGG CAA TAT TAT C	1394–1411	45	Sequencing
	maropR-r1950	GTC CAA AAT GCT ATG AAT G	1950–1928	45	Sequencing
	marop-f1140	GCCAGGCCAAGAAATAAC	1140–1157	50	Sequencing
	marop-r1556	ATCCAGCGGAGACAGATAC	1556–1538	50	Sequencing
marA, marB	marAB-f1840	GGC AAC ACT TGA GTA TTT GC	1840–1859	50	Sequencing
	marAB-r2704	AGT AGG ACT GGC AAG TGC	2704–2687	50	Sequencing
marA	marA-f1893	ATT AAG CTT ATG ACG ATG TCC AGA CG	1893–1909	50	HindIII site
	marA-r2281	ATT TCT AGA ACT AGC TGT TGT AAT G	2282–2267	50	XbaI site
marR	marR-f1452	AAT GAA TTC GTA CCA GCG ATC TGT TCA ATG	1452–1472	50	EcoRI site
	marR-r2229	AAT CTG CAG TGG TCA TCC GGT ATT TAT GC	2229–2210	50	PstI site
soxS	soxS-f331	ATA GAA TTC ACC AAT AAA ATT ACA GGC GG	3331–3350	50	EcoRI site
	soxS-r1319	ATA CTC GAG ATG TCC CAT CAG AAA ATT ATT CAG	1319–1297	50	XhoI site
acrA	acrA-f31	GCG GTC GTT CTG ATG CTC TC	31–48	50	Northern probe
	acrA-r330	ACC TTT CGC ACT GTC GTA TG	330–310	50	Northern probe

^aRestriction sites are italicized. 

^bT_an, annealing temperature.