TABLE 2.
Bacterial strains and plasmids used in this study
| E. coli strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| ATCC 25922 | E. coli | ATCC |
| EP1 | MIC of CIP, 16 mg/liter | Clinical isolate |
| EP2 | MIC of CIP, 256 mg/liter | Clinical isolate |
| EP2 acrA::Tn10-Km | Derivative of isolate EP2 in which acrA was inactivated by transposon mutagenesis | This study |
| S17 λpir + pLOF/Km | Suicide vector for insertion mutagenesis | 10 |
| S17 + p591 | Wild-type marR in trans | Hüllen et al., 37th ICAAC |
| Plasmids | ||
| pmarR-ΔC18 | Derived from pUC18 containing the PCR product of primers marR-f1452 and marR-r2229 | This study |
| pc-marA | Derived from pcDNA3 containing the PCR product of primers marA-f1893 and marA-r2281 | This study |
| pc-soxS | Derived from pcDNA3 containing the PCR product of primers soxS-f331 and soxS-r1319 | This study |