Figure 4.

Expression levels of AcWRKY genes isolated in onion bulb at the beginning of dormancy (T₁), late dormancy (T₂), and the dormancy release (T₃). (A) Heatmap developed using log2 FC values (by Deseq2) of AcWRKYs for uninfected (U)/OYDV-infected (I) bulbs compared at each time; AcWRKYs showing significant differences by RNASeq (p < 0.05; log2 FC > 1) and qPCR between the two conditions during dormancy release (T₃) were underlined. (B) Normalized RNA-Seq counts of AcWRKY2, 30, and 32 at each sampling time, detected in uninfected (red) and OYDV-infected (gray) samples. ** p < 0.05, * p < 0.1 (between infected and uninfected bulbs).