FIG. 1.
Nucleotide sequence of a 2,384-bp DNA fragment of recombinant plasmid pBS2 carrying blaGOB-1 and the 117 bp of the 3′ end of the endo-beta-N-acetylglucosaminidase gene of C. meningosepticum PINT. The deduced amino acid sequences are given in a single-letter code. The start and stop codons of the blaGOB-1 gene and the stop codon of the endo-beta-N-acetylglucosaminidase gene are in bold. The vertical arrow indicates the peptide leader cleavage site in E. coli as determined by N-terminal sequencing. The putative −35 and −10 sequences of the putative promoter and ribosome binding site (RBS) for blaGOB-1 are underlined. Primers 1, 2, and 3 used to PCR amplify blaGOB-1-like genes from other C. meningosepticum isolates are indicated by an arrow.