TABLE 4.
pI values of β-lactamase activity detected in C. meningosepticum isolates and in E. coli DH10B harboring pBS2 (GOB-1) or pBS4 (BlaB-1) and the corresponding GOB-1-like and BlaB-1-like sequences
| Strains | pI values
|
Shared amino acid sequence
|
||
|---|---|---|---|---|
| Clavulanic acid-inhibited β-lactamase activity | EDTA-inhibited β-lactamase activity | GOB-1 like | BlaB-1 like | |
| C. meningosepticum PINT | 7.6 | 8.3 | GOB-1 | BlaB-1 |
| C. meningosepticum CIP 6058 | 8.5 | 7.8, 8.7 | GOB-5 | BlaB-1 |
| C. meningosepticum AMA | 8.5 | 8.3 | GOB-6 | BlaB-2 |
| C. meningosepticum GEO | 7.0 | 7.5, 8.5 | GOB-4 | BlaB-3 |
| C. meningosepticum CIP 7830 | 7.5 | 8 | +a | BlaB-6 |
| C. meningosepticum CIP 6059 | 8.4 | 7.5, 8.5 | GOB-3 | BlaB-3 |
| C. meningosepticum CIP 79.5 | 7.6 | 8.4 | GOB-7 | BlaB-5 |
| C. meningosepticum AB1572 | 7.6 | 8.3 | GOB-1 | BlaB-7 |
| C. meningosepticum H01J100 | 8.1 | 8.3 | GOB-2 | BlaB-8 |
| C. meningosepticum CIP 6057 | 7.6 | 8.6 | GOB-1 | BlaB-4 |
| E. coli DH10B (pBS2) | —b | 8.7 | GOB-1 | — |
| E. coli DH10B (pBS4) | — | 8.5 | — | BlaB-1 |
a
+, GOB-1-like positive results by hybridization but negative by PCR amplification.
b
—, not detectable.