Figure 1.

L-type Ca²⁺ channels inhibition completely reversed the ISO-induced acceleration of depolarization rate in both dormant and spontaneously firing SANC isolated from WT mice. (A) Proportion of dormant and spontaneously firing SANC at baseline. (B) Current-clamp recording sample of dormant SANC initiating firing when injected with hyperpolarizing current. Average depolarization rates and illustrative examples of dormant ((C–E), n = 8) and firing ((D–F), n = 10) SANC at baseline and after perfusion of isoprenaline (ISO 100 nM) and subsequent perfusion of nifedipine (3 µM). * p < 0.05, ** p < 0.01, **** p < 0.0001 by parametric one-way ANOVA or non-parametric Friedman test.