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LCR regulation by Cav1.3 Ca2+ channels is not due to a decrease in phosphorylated PLB levels. Double immunostaining of phosphorylated PLB (pPLB) at serine 16 (green), total PLB (red) and merged images in SANC incubated with Tyrode (n = 30), ISO 100 nM (n = 32) or ISO + Nife 3 µM (n = 45) with mean pPLB/PLB ratio (bottom). ns: non-significant, **** p < 0.0001 by one-way ANOVA test.
