Table 2.
Solvent gradient system used for phospholipids class HPLC separation.
| Gradient System for Phospholipids Class Separation | ||||
|---|---|---|---|---|
| Time (min) | Flow Rate (mL/min) | Hexane (%) | Isopropanol (%) | Water (%) |
| 0.01 | 0.8 | 53 | 42 | 5 |
| 5 | 1 | 40 | 55 | 5 |
| 10 | 1.25 | 40 | 50 | 10 |
| 30 | 1.25 | 40 | 55 | 5 |
| 35 | 0.8 | 53 | 42 | 5 |