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. 2022 Mar 24;11(7):1087. doi: 10.3390/cells11071087

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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HLH4 is localized in the nucleus and is predominantly expressed in seedlings and reproductive organs. (A) Genome organization of HLH4 and synteny with its paralog HLH3 on A. thaliana chromosomes. (B) Protein sequence alignment of HLH4 and HLH3. Helix-loop-helix structure domains (https://www.predictprotein.org/, accessed on 21 March 2022) are indicated, and the dashed box represents hypothetical basic regions. (C) Subcellular localization of HLH4-GFP fusion protein in A. thaliana protoplasts. (D) RT-qPCR analysis of HLH4 expression from various tissues. Data represent mean ± SD of three biological replicates with three technical replications (n = 9). (E) In vivo analysis of the HLH4 expression pattern using proHLH4::GUS reporter lines.