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. 2022 Mar 25;11(7):1106. doi: 10.3390/cells11071106

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of Doxo stimulation on the expression of key senescence regulators CDKN1A and CDKN2A. Gene expression analysis of (A) CDKN1A (p21) and (B) CDKN2A (p16) in hAC after 10 d of Doxo stimulation at different concentrations; unstimulated hAC served as controls. (C) Exemplary images of a CDKN2A immunostaining in hAC after stimulation with 0.1 µM Doxo for 7 d. Data are presented as box plots with median, whiskers min to max. Significant differences between groups are depicted as: **** p ≤ 0.0001; *** p ≤ 0.001; ** p ≤ 0.01; * p ≤ 0.05. Statistical analysis: (A,B) One-way ANOVA, Sidak’s multiple comparison. Scale bar = 50 μm. Ctrl = control (unstimulated cells), dapi = 4′,6-diamidino-2-phenylindole.