Table 2.

Analytical conditions for the determination of the tested analytes in various matrices.

Condition	Content
Instrument	LC: Alience 2695 LC separation module, Waters MS/MS: Micromass Quattro Micro triple quadrupole tandem mass, Waters
Chromatographic separation
Column	Agilent ZORBAX Eclipse Plus C18 (3.0 mm I.D. × 150 mm L, 3.5 μm)
Flow rate	0.3 mL/min
Injection volume	5 μL
Oven temp.	40 °C
Mobile phase	A: 5 mM ammonium formate, 0.1% formic acid in methanol B: 5 mM ammonium formate, 0.1% formic acid in water
Gradient	Time	A (%)	B (%)
	0.0	5	95
	2.0	5	95
	4.0	95	5
	10.0	95	5
	12.0	5	95
	15.0	5	95
MS/MS condition
Capillary voltage	3.9 kV
Source temp.	150 °C
Desolvation gas temp.	250 °C
Desolvation gas flow	500 L/h