Table 1.
Primer sequences used in the study.
| Gene Name | Forward Primer Sequence (5′–3′) | Reverse Primer Sequence (5′–3′) |
|---|---|---|
| ACAN | AAGAATCAAGTGGAGCCGTGTGTC | TGAGACCTTGTCCTGATAGGCACT |
| COL2A1 | AAGGTGCTTCTGGTCCTGCTG | GGGATTCCATTAGCACCATCTTTG |
| TNFA | GTGACAAGCCTGTAGCCCATGTT | TTATCTCTCAGCTCCACGCCATT |
| IL-6 | AAGCCAGAGCTGTGCAGATGAGTA | TGTCCTGCAGCCACTGGTTC |
| MMP-3 | CAAGGAGGCAGGCAAGACAGC | GCCACGCACAGCAACAGTAGG |
| ADAMTS-4 | GGATTACAGGTGTGAGCCACCA | GGATGCAACCACATCTGTCTGA |
| GAPDH | GAGGCCGGTGCTGAGTAT | GCGGAGATGATGACCCTTTTGG |
ACAN = aggrecan; ADAMTS = a disintegrin and metalloproteinase with thrombospondin motifs; COL2A1 = collagen type II alpha 1 chain; GAPDH = glyceraldehyde 3 phosphate dehydrogenase; IL = interleukin; MMP = matrix metalloproteinase; TNFA = tumor necrosis factor-α.