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. 2022 Apr 2;11(7):1196. doi: 10.3390/cells11071196

Figure 1.

Figure 1

Changes in TRPV2 protein expression. (a,b) Representative images (a) and summary data (b) showing TRPV2 expression levels in human lens epithelial cells from patients having senile cataracts (SC) or diabetic cataracts (DC). In (b), data showing the ratio of integrated optical density/total area of TRPV2. (c) Immunofluorescence images showing the expression of connexin-46 (Con-46) protein in primary cultured lens epithelial cells from rat. No—primary antibody control: I–III Connexin-46 expression: IV-VI. Cell nuclei are stained blue by 4′,6-diamidino-2-phenylindole (DAPI). (d–g) Representative Western blotting images (d,f) and summary data (e,g) showing the expression levels of TRPV2 in HLEpiCs (d,e) and primary rat lens epithelial cells (f,g) cultured in a normal-glucose (NG, 5.5 mM glucose and 20 mM mannitol) or high-glucose (HG, 25.6 mM glucose) media for 1, 3, 5, and 7 days. Numbers in parentheses over the bars in panels (e,g) represent the number of biological replicates. Values are shown as the mean ± SEM; n = 3–11. * p < 0.05 vs. SC or NG at 3, 5, and 7 days analyzed by two-tailed unpaired Student’s t test; ns, not significant.