Figure 2.

Changes of TRPV2-mediated Ca²⁺ influx in a high-glucose environment. (a–f) Representative traces (a,b,d,e) and summary data (c,f) showing the changes in the intracellular Ca²⁺ concentration of HLEpiCs (a–c) and primary rat lens epithelial cells (d–f) cultured in a normal-glucose (NG, 5.5 mM glucose and 20 mM mannitol) or high-glucose (HG, 25.6 mM glucose) medium for seven days. The cells were treated without or with ruthenium red (RR, 10 μM) and activated by 2-aminoethyldiphenyl borate (2-APB, 250 μM). (g) Summary data showing TRPV2 mRNA expression level of HLEpiCs transfected with TRPV2 siRNA or scrambled siRNA control for three days. (h–i) Representative Western blotting images (h) and summary data (i) showing TRPV2 protein expression levels of HLEpiCs transfected with TRPV2 siRNA or scrambled siRNA control for three days. (j–k) Representative traces (j) and summary data (k) of the changes in intracellular Ca²⁺ concentration of HLEpiCs transfected with TRPV2 siRNA or scrambled siRNA control and cultured in NG or HG media for seven days. The TRPV2 channel agonist 2-APB (250 μM) activated the channel to induce Ca²⁺ influx. Values are shown as the mean ± SEM; n = 3–6. * p < 0.05 analyzed by two-way analysis of variance followed by a Bonferroni test in panel c, f and k, and two-tailed unpaired Student’s t test in panel (g,i); ns, not significant.