Figure 5.

Time-dependent expression of β-catenin in the ex vivo wounds. (A) Immunofluorescence staining of β-catenin protein expressed in the epidermis close to the wound edges. Representative images of stained cryosections (β-catenin in green, PI-stained cell nuclei in red, and overlay) for each group and time point. White arrow points to ASCs. All pictures were taken with the same magnification; scale bar, 20 μm. (B) Representative immunostained sections of newly formed epithelia at the edge and in the center of wounds at day 12. The top of the wound is indicated with a continuous line while the newly formed epithelia is indicated by dashed lines. All pictures were taken with the same magnification; scale bar, 20 μm. D, dermis (C) Analysis of the fluorescence intensity of at least 15 random fields per time point from three (control) or six donors (samples). The mean fluorescence intensity (MFI) was measured using the ImageJ software. Data are shown as median with interquartile range (IQR). The statistical analysis was performed using the Mann–Whitney U test. * p < 0.05.