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. 2022 Mar 22;11(7):1071. doi: 10.3390/cells11071071

Figure 2.

Figure 2

LPA5 deletion attenuates expression of pro-inflammatory genes in mouse brain. Wt and LPA5-/- mice were injected i.p. with PBS (n = 4) or LPS (5 mg/kg; n = 6). After 24 h, the animals were sacrificed and perfused. Brains were processed for RNA isolation and gene expression of (A) TNFα, (B) IL-6, (C) IL-1β, (D) CXCL10, (E) CXCL2, (F) CCL5, (G) iNOS, and (H) Arg-1 was evaluated by qPCR. Hypoxanthine-guanine phosphoribosyltransferase (HPRT) was used as housekeeping gene. Expression was calculated using the 2−ΔΔCt method. Results are presented as mean values ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001 compared to wt PBS control; & p < 0.05, && p < 0.01 compared to LPA5-/- PBS control; # p < 0.05 compared to LPS-treated wt mice; two-way ANOVA with Bonferroni correction).