Figure 1.

Recombination efficiency in retinal microglia and blood of Vldlr^−/−; MG^Tom mice. (A) Scheme displaying the experimental setup and timeline (left-hand side panel) for induction of reporter expression (right-hand side panel). Newborn Vldlr^−/−; MG^Tom mice were injected with tamoxifen (TAM) on postnatal day (p)1. Flow cytometry (FACS) and immunohistochemistry (IHC) were performed at p5 and p12. (B) Immunofluorescence of retinal vessels (Collagen IV (COLIV) staining, blue) and microglia (IBA1, green) at p5 and p12 following tamoxifen treatment at p1. TAM injection at p1 leads to efficient tdTomato (TOM, red) labeling of most microglia. In the top right corner of each image, a magnified image of the area within the dashed white box is displayed. (C) Flow cytometry analysis of the recombination efficiency in monocytes of Vldlr^−/−; MG^Tom mice at p5 and p12. Monocytes were gated as a CD45^hiCD11b⁺CD115⁺Ssc^lo cell population from peripheral blood and analyzed for the proportion of TOM⁺ cells. FSC = forward scatter; SSC = sideward scatter. (D) Immunofluorescence-based quantification of TOM⁺ IBA1⁺ microglia of TAM-treated Vldlr^−/−; MG^Tom mice at p5 and p12. P5: n = 17, p12: n = 14 mice. (E) Flow cytometry-based quantification of TOM⁺ monocytes in the blood of Vldlr^−/−; MG^Tom mice. Data are presented as mean ± SD. p5: n = 6, p12 n = 5 mice.