Figure 7.

Phenotypic characterizations of knockout lines. (A) Deletion of nucleotides through CRISPR/cas9 in ko-1, ko2 and ko3, (B) The phenotype of KO lines displaying lesion mimic spots on leaves at tillering stage. NIP: Nipponbare. Scale bar: 1 cm. (C) Changes of CAT activity in the leaf of NIP and ko1 at the tillering stage, (D) Comparison of H₂O₂ content in the leaves between NIP and ko1 at the tillering stage, (E) Sub-cellular structure of leaves in NIP at the end of the night, (F) Sub-cellular structure of leaves in ko1 at the end of the night, (G) Sub-cellular structure of leaves in NIP at the end of the day, (H) Sub-cellular structure of leaves in ko1 at the end of the day, where CW, cell wall, CH, chloroplast; SG, starch granules; OB, osmiophilic body. Scale bar: 2 μm in (E–H). Comparison of agronomic traits e.g plant height (I), tiller number (J), number of grains per panicle (K), 1000-grains weight (L), and seed setting rate (M). Data were obtained from 10 plants of NIP and ko1. Statistical analysis was performed using Student’s t-test, where *** indicates p < 0.001 in (D) and **** indicates p < 0.0001 in (C,I–M). Data presented in (I–M) were the average of n = 3 plants.