TABLE 2.
ClaI::mecA vicinity organization features of MRSA epidemic clonesa
| Strain | Pattern | Organization featuresb | Observation/origin (reference) |
|---|---|---|---|
| HUC19 | I | ΔHVR + pUB110 + Ins117 | Iberian clone/Portugal (21) |
| HUC31 | I | ΔHVR + pUB110 + Ins117 | Iberian clone/Portugal (21) |
| BK1920 | I | ΔHVR + pUB110 + Ins117 | Iberian clone/USA (22) |
| BK59 | I | ΔHVR + pUB110 + Ins117 | mecA type I control strain (16) |
| BK2464 | I | pUB110 + Ins117 | New York clone/USA (17) |
| JP1 | I | pUB110 + Ins117 | Epidemic clone/Japanc |
| PL72 | I | pUB110 | Epidemic clone/Poland (18) |
| COL | II | Reference strain/RU collection (8) | |
| BB270 | II | (24) | |
| BK71 | II | mecA type II control strain (16) | |
| HUC191 | II | ΔHVR + Ins117 | Iberian clone related/Portugal (21) |
| DEN 2125 | II | Epidemic clone 1960s/Denmark (4) | |
| R35 | III | pT181 + pI258 | Reference strain/USA (13) |
| CPS22 | III | pT181 | Epidemic clone/Portugal (5) |
| CPS68 | III | pT181 | Epidemic clone/Portugal (5) |
| HU25 | III* | pI258 | Brazilian clone (29) |
| PER168 | IX | pT181 + pI258 | mecA type IX control strain (12) |
| PER222 | XI | pT181 + pI258 | mecA type XI control strain (12) |
| BK163 | III | pT181 + pI258 | mecA type III control strain (16) |
| HU106 | IX | pT181 + pI258 | Hungarian cloned (6) |
| HU41 | XI | pT181 + pI258 | Hungarian cloned (6) |
| HU101 | III | pT181 + pI258 | Hungarian cloned (6) |
| HU86 | III′ | pT181 + pI258 | Hungarian cloned (6) |
| BK2421 | IV | mecR1Δ170bp (5′)/pT181+pI258 (3′) | mecA type IV control strain (16) |
| LHH1 | IV | mecR1Δ170bp (5′)/pT181+pI258 (3′) | Epidemic clone/USA (7) |
| BK2529 | V | IS256 | mecA type V control strain (16) |
| BARGII17 | V | IS256 | Epidemic clone/USA (7) |
| HSA49 | V | IS256 | Sporadic clone/Portugal (10) |
| BK793 | VI | pI258 | mecA type VI control strain (16) |
| HU195 | VI | pI258 | Sporadic clone/Hungary (5) |
| HUC64 | VIII | ΔHVR + pUB110 + IS256 | Iberian clone related/Portugal (21) |
| IPO108 | XIII | ΔHVR + pUB110 + IS256 | Iberian clone related/Portugal (26) |
| VIC1 | XIV | ΔHVR + IS256 | Iberian clone related/Portugale |
| PL46 | XV | mecR1::IS256 (5′)/pT181 (3′) | Sporadic clone/Poland (18) |
The small differences among strains within pattern I or II (ΔHVR and Ins117) cannot be detected by the conventional gel electrophoresis settings used in ClaI::mecA pattern determination (5, 16). Polymorphisms IX, XI, III, and III′ differ primarily in the copy number of the short 40-bp sequence within the dru element: 11 copies in IX, 10 in XI, 9 in III, and 8 in III′ (see accession numbers in Materials and Methods).
Abbreviations: ΔHVR, truncated HVR, which has a deletion of 1,043 bp spanning all of the dru element; Ins117, 117-bp sequence flanked by two 15-bp direct repeats; mecR1Δ170bp, 170-bp deletion in the mecR1 coding region.
M. A. de Sousa et al., unpublished data.
D. Oliveira, P. Major, I. S. Sanches, A. Marton, and H. de Lencastre, Abstr. 38th Intersci. Conf. Antimicrob. Agents Chemother., abstr. E170, 1998.
R. Sá-Leão, I. S. Sanches, F. Fonseca, and H. de Lencastre, Abstr. 1st Eur. Congr. Antimicrob. Agents Chemother., abstr. F164, 1996.