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. 2022 Mar 29;23(7):3734. doi: 10.3390/ijms23073734

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Proteasome inhibitor-induced reorganization of the microtubule structure in neuronal somata. Sensory neurons were differentiated for at least 38 days after thawing and exposed to bortezomib, carfilzomib, or taxol for 24 h before fixation. (A) Representative immunofluorescence images of cells stained for βIII-tubulin. A scale bar is given in the images, and further details are shown in Figure S11. (B) Cells exhibiting intense, circular βIII-tubulin staining around the cell somata (covering at least 50% of a full circle) were quantified. Data are given as % of the total cell count (number of viable cell nuclei) and are expressed as the mean ± SD of 2–3 biological replicates. *, p < 0.05; ***, p < 0.0001.