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. 2022 Mar 22;23(7):3442. doi: 10.3390/ijms23073442

Figure 6.

Figure 6

Validation of the results of the RNA-seq using quantitative real-time PCR with 5 chosen genes analyzed by the ΔΔCt method, shown as absolute fold induction relative to infected, normalized to GAPDH, * p < 0.05, ** p < 0.01 (n = 3), A = gene expression after 4 h, B = gene expression after 24 h. Expressions of the following genes were analyzed: CCL20 = chemokine (C-C motif) ligand 20 (CCL20), CXCL8 = chemokine (C-X-C motif) ligand 8, NFKBIA = nuclear factor kappa B inhibitor alpha, TNFAIP3 = tumor necrosis factor alpha-inducible gene 3 protein, TRAF5 = TNF receptor-associated factor 5, CYP1A1 = cytochrome P450 family 1 subfamily A member 1, NOD2 = nucleotide-binding oligomerization domain-containing 2.