Figure 1.

Agm effect on viability and NO production of control and Lps-stimulated BV-2 cells. If not stated otherwise, for these and analyses presented in the following figures, BV-2 cells were seeded at a density of 3.4 × 10⁴ cells/cm². Cells were pretreated with agmatine sulfate—Agm (1, 10, and 100 µm) for 30 min and then stimulated with Lps (1 µg/mL) for additional 24 h. (a) Cell viability of control and Lps-stimulated BV-2 cells treated with Agm. (b) Modulation of Lps-induced production of NO with Agm. The dashed line represents the limit of detection of the Griess assay (~2.5 μm). Results are expressed as the mean ± SEM (n = 3) and were analyzed by Kruskal–Wallis test followed by Dunn’s multiple comparisons tests; the groups not sharing a joint letter are significantly different (p < 0.05).