TABLE 3.
Membrane sterol levels in K. lactis and K. lactis mutant strains grown in the presence or absence of AmBa
| Compound | Relative retention timeb | % of sterol content in strain
|
|||
|---|---|---|---|---|---|
| K. lactis | K. lactis + AmB | K. lactis mutant | K. lactis mutant + AmB | ||
| Cholesterol | 1 | 1 ± 0.2 | 3 ± 0.4 | 5 ± 0.4 | 6 ± 0.5 |
| Dehydroergosterol | 1.08 | 2 ± 0.3 | 5 ± 0.5 | 2 ± 0.4 | 8 ± 0.7 |
| Zymosterol | 1.12 | 3 ± 0.6 | 12 ± 1.0 | 9 ± 0.8 | 13 ± 1.1 |
| Dihydroergosterol | 1.20 | 5 ± 0.5 | 17 ± 1.5 | 3 ± 0.4 | 5 ± 0.6 |
| Ergosterol | 1.23 | 81 ± 4 | 37 ± 4 | 65 ± 3 | 44 ± 5 |
| 4-Methylzymosterol | 1.27 | 2 ± 0.4 | tr | 12 ± 0.9 | 7 ± 0.7 |
| Fecosterol | 1.28 | tr | 3 ± 0.3 | tr | 2 ± 0.3 |
| Stigmasterol | 1.35 | tr | 2 ± 0.3 | tr | tr |
| Episterol | 1.37 | 2 ± 0.2 | 5 ± 0.3 | 1 ± 0.1 | 7 ± 0.6 |
| Ergosta-7-ene-3-ol | 1.40 | tr | tr | tr | 2 ± 0.2 |
| Lanosterol | 1.45 | 2 ± 0.3 | 9 ± 1.3 | 1 ± 0.2 | 5 ± 0.5 |
| 4,4-Dimethylzymosterol | 1.51 | tr | 6 ± 0.5 | 1 ± 0.2 | tr |
| 4,4-Dimethyl-ergosta-8,24(28)diene-3-ol | 1.73 | 1 ± 0.2 | tr | tr | tr |
a
AmB was used at subinhibitory doses (10 and 125 mg/liter for K. lactis and the K. lactis mutant, respectively).
b
Retention times are expressed as ratio to cholesterol retention time (1).