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. 2022 Mar 28;23(7):3709. doi: 10.3390/ijms23073709

Figure 6.

Figure 6

ELK3 regulates gastric cancer cell adhesion through bone morphogenetic protein1 (BMP1)-mediated ECM remodeling. (A) Expression of mRNA encoding the ELK3 and BMP1 genes in SNU638 cells transfected with shCon, shELK3, shELK3 plus pLenti-cMyc-DDK-ELK3 plasmid DNA (ELK3 rescue), or ELK3 rescue plus siBMP1. Error bars represent the standard deviation; * p < 0.05, *** p < 0.001, and **** p < 0.0001 (one-way ANOVA). (B) Expression of mRNA encoding the LOXL2, SNAI1, SERPINF1, DCN, and NID1 genes in SNU638 cells transfected with shCon, shELK3, ELK3 rescue, or ELK3 rescue plus siBMP1. Error bars represent the standard deviation; ** p < 0.01, *** p < 0.001, and **** p < 0.0001 (one-way ANOVA). (C) Representative images showing cell adhesion of SNU638 cells transfected with shCon, shELK3, ELK3 rescue, or ELK3 rescue plus siBMP1. Scale bar, 200 μm. Bar graphs indicate the absorbance value of adherent cells. Error bars represent the standard deviation; ** p < 0.01 and *** p < 0.001 (one-way ANOVA). (D) Representative images showing cell migration and invasion of SNU638 cells transfected shCon, shELK3, ELK3 rescue, or ELK3 rescue plus siBMP1. Scale bar, 200 μm. Bar graphs indicate the number of migrated cells. Error bars represent the standard deviation; * p < 0.05 and ** p < 0.01 (one-way ANOVA). ELK3, ETS transcription factor ELK3; sh, short hairpin RNA; Con, control.