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. 2022 Mar 23;23(7):3511. doi: 10.3390/ijms23073511

Figure 7.

Figure 7

Effect of sTN145, sTN58 and sTN29 aptamers on mammosphere-forming efficiency of MDA-MB-231 cells. (A) Viability of MDA-MB-231 cells mock-treated or treated with the indicated aptamers for 24 h. Data are expressed as a percentage of viable treated cells with respect to mock-treated cells. (B) Representative phase-contrast images of MDA-MB-231 cells, previously treated for 24 h with sTN145, sTN58, sTN29 or Scr, after 10 days of growth in stem-permissive conditions. Magnification 10×, scale bar = 200 μm. Cell treatment with the short TNBC aptamers, but not the Scr, inhibits both the number, expressed as a percentage with respect to mock-control cells (left) and diameter (right) of mammospheres. (C) Mammosphere self-renewal capacity is expressed as a number of secondary mammosphere population (P1)/cell-seeded × 100. (A–C) Bars depict mean ± SD of three independent experiments. In (B,C), *** p < 0.001; * p < 0.05 relative to mock-control cells. No statistically significant variations between Scr and mock-treatment were obtained.