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. 2022 Mar 28;23(7):3702. doi: 10.3390/ijms23073702

Figure 4.

Figure 4

Effect of MCPyV LT and sT on the activity of IL-33 promoter mutants. (A–C) MCC-13 cells were co-transfected with a luciferase reporter plasmid containing the human IL-33 promoter encompassing sequences −1050 to +50 or truncated version, together with an empty expression vector pcDNA3.1 (EV) or an expression of plasmid encoding MCPyV full-length LT (A), LTMKL-2 (B) or sT (C). (D,E) MCC-13 cells were co-transfected with a luciferase reporter plasmid containing IL-33 promoter sequences −1050/+50, or with a mutation of either one or both potential LT binding sites, together with empty vector, LT (D) or LTMKL-2 (E). Mutated sequences are indicated in red. Luciferase values were normalized to the total protein concentration in the sample. Each bar represents the average ± SD of three independent parallels. Each experiment was performed 2 to 4 times, and similar results were obtained. * p < 0.05, ** p < 0.01; *** p < 0.001.