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. 2022 Mar 24;23(7):3544. doi: 10.3390/ijms23073544

Figure 3.

Figure 3

Kaempferol promotes 5-Fu sensitivity by inhibiting glycolysis. (A) Analysis of glucose content in HCT8 and HCT8-R cell culture media. (B) The content of lactate was detected in HCT8 and HCT8-R cell culture media. (C) After HCT8 and HCT8-R cells were cultured in glucose deprivation medium containing 10%, 20%, 50% and 100% glucose for 24 h, a CCK-8 assay was used to detect cell survival. Data represent means ± SD from three independent experiments. (D) After HCT8 and HCT8-R cells were treated with various concentrations of 2-DG (2-deoxy-d-glucoside) (0 mM, 0.5 mM, 1 mM, 2 mM, 4 mM, 8 mM,12 mM and 16 mM) for 48 h, a CCK-8 assay was used to detect cell survival. Data represent means ± SD from three independent experiments. (E) After HCT8-R cells were treated with kaempferol for 48 h, the content of glucose in the culture medium was detected. (F) After HCT8-R cells were treated with kaempferol for 48 h, the content of lactate in the culture medium was detected. * p < 0.05, ** p < 0.01.