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. 2022 Mar 24;23(7):3544. doi: 10.3390/ijms23073544

Figure 4.

Figure 4

PKM2 mediates kaempferol, increasing the HCT8-R’s sensitivity to 5-Fu. (A) The protein expression of PKM2 (pyruvate kinase M2 isoform) and hnRNPA1/A2 (heterogeneous nuclear ribonucleoprotein A1/A2)/PTBP1 (polypyrimidine-tract-binding protein) in HCT8 and HCT8-R cells was analyzed by Western blot. (a) A representative result from three independent experiments is shown. (b,c) The densitometry analysis of relative protein expression is shown in a histogram. (B,C) qPCR was used to analyze the expression of PKM2 mRNA in HCT8-R cells after knockdown (B) and over-expression (C) of PKM2. (D) Western blot was used to analyze the expression of PKM2 protein in HCT8-R cells after overexpression or knockdown of PKM2. (a) A representative result from three independent experiments is shown. (b) The densitometry analysis of relative protein expression is shown in a histogram. (E) After transfecting with shNC or shPKM2, the HCT8-R cells were cultured in medium containing kaempferol for 48 h, and a CCK-8 assay was used to detect cell survival. (F) After transfecting with Flag or Flag-PKM2, the HCT8-R cells were cultured in medium containing kaempferol for 48 h, and a CCK-8 assay was used to detect cell survival. Data represent means ± SD from three independent experiments. * p < 0.05, ** p < 0.01, **** p < 0.0001.