. 2022 Mar 24;23(7):3547. doi: 10.3390/ijms23073547

Table 2.

Sequence-dependent characteristics of LEAPs from R. serbica. The physicochemical parameters of each LEAP were calculated by using the ExPASy online server (http://web.expasy.org/compute_pi/, accessed on 8 February 2022) and by the GRAVY calculator (http://www.gravy-calculator.de/, accessed on 8 February 2022).

LEA Protein Group	aa #	Calculated pI	Mw (kDa)	GRAVY Index	Amino Acid (aa) Composition
					%	%	%	%	%
					Charged	Polar	Nonpolar	Aromatic	Cys
LEA1	139 ± 5	8.2 ± 0.4 ^d	14.4 ± 0.5 ^a	−0.93 ± 0.05 ^b	23.2 ± 1.3 ^a	33.2 ± 0.9 ^c,d	40.5 ± 0.8 ^c	5.1 ± 0.5 ^a	0.10 ± 0.06 ^a
LEA2	226 ± 5	8.4 ± 0.3 ^d	25.2 ± 0.7 ^b	−0.09 ± 0.03 ^e	21.4 ± 0.5 ^a	29.3 ± 0.5 ^b,c	38.8 ± 0.4 ^b,c	9.7 ± 0.2 ^c	1.66 ± 0.10 ^c
LEA3	126 ± 5	7.0 ± 0.4 ^b,c,d	14.0 ± 0.6 ^a	−0.59 ± 0.03 ^c	23.6 ± 0.5 ^a	35.6 ± 1.0 ^d	32.3 ± 0.8 ^a	10.5 ± 0.4 ^c	0.56 ± 0.09 ^a,b
LEA4	187 ± 7	6.1 ± 0.2 ^a,b	17.9 ± 0.9 ^a	−1.01 ± 0.03 ^b	35.2 ± 0.6 ^b,c	23.6 ± 0.5 ^a	36.7 ± 0.5 ^b	3.7 ± 0.2 ^a	0.33 ± 0.06 ^a,b
LEA5	118 ± 7	8.1 ± 0.5 ^c,d	12.7 ± 0.8 ^a	−1.02 ± 0.14 ^b	30.0 ± 1.4 ^b	29.2 ± 1.7 ^b,c	36.9 ± 1.7 ^b,c	4.7 ± 0.9 ^a	0.56 ± 0.28 ^a,b
Dehydrin	143 ± 9	6.7 ± 0.5 ^b,c	15.6 ± 1.0 ^a	−1.40 ± 0.05 ^a	37.2 ± 2.7 ^c	28.7 ± 1.5 ^b,c	29.3 ± 1.5 ^a	9.5 ± 0.5 ^c	1.00 ± 0.19 ^b,c
SMP	157 ± 15	4.9 ± 0.3 ^a	16.4 ± 1.6 ^a	−0.27 ± 0.04 ^d	22.9 ± 0.8 ^a	26.9 ± 1.3 ^a,b	46.6 ± 1.0 ^d	6.8 ± 0.4 ^b	0.88 ± 0.21 ^b

Values represent the mean ± SE; different letters denote statistically significant differences between different LEA protein family groups (p < 0.05).