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. 2022 Mar 29;23(7):3733. doi: 10.3390/ijms23073733

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Crosstalk between necroptosis, autophagy, and apoptosis after mitotic catastrophe induction. (A) Caov4 and HCT116 cells were treated with 600 nM doxorubicin and 40 μM zVAD-fmk, and/or 30 μM necrostatin-1s, and/or 25 nM bafilomycin A1 for 48 h. Immunoblot analysis using the indicated antibodies is shown. (B) Sub-G1 analysis of Caov4 and HCT116 cells treated with 600 nM doxorubicin and 40 μM zVAD-fmk, and/or 30 μM necrostatin-1s, and/or 25 nM bafilomycin A1 for 48 h. Values are the mean (±standard deviation of the mean) of three independent experiments. (C) U1810 wild type and ATG13-knockout cells were treated with 600 nM doxorubicin and 40 μM zVAD-fmk and/or 30 μM necrostatin-1s for 24 h. Immunoblot analysis using the indicated antibodies is shown. * p < 0.05, ** p < 0.01.

Crosstalk between necroptosis, autophagy, and apoptosis after mitotic catastrophe induction. (A) Caov4 and HCT116 cells were treated with 600 nM doxorubicin and 40 μM zVAD-fmk, and/or 30 μM necrostatin-1s, and/or 25 nM bafilomycin A1 for 48 h. Immunoblot analysis using the indicated antibodies is shown. (B) Sub-G1 analysis of Caov4 and HCT116 cells treated with 600 nM doxorubicin and 40 μM zVAD-fmk, and/or 30 μM necrostatin-1s, and/or 25 nM bafilomycin A1 for 48 h. Values are the mean (±standard deviation of the mean) of three independent experiments. (C) U1810 wild type and ATG13-knockout cells were treated with 600 nM doxorubicin and 40 μM zVAD-fmk and/or 30 μM necrostatin-1s for 24 h. Immunoblot analysis using the indicated antibodies is shown. * p < 0.05, ** p < 0.01.