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. 2022 Mar 23;23(7):3495. doi: 10.3390/ijms23073495

Figure 4.

Figure 4

Oligomerization reduces the binding of Tau to Nup98-FG. (A) DLS size distribution profiles for Tau, P-Tau, and P-TauΔK280 at 0 h (blue) and 48 h (purple) of the oligomerization process (spontaneous oligomerization at room temperature). Both P-Tau and pro-aggregant P-TauΔK280, but not Tau, show a shift from Tau monomers (radius ~15 nm) at 0 h towards oligomers (radius ~600 nm) at 48 h. (B) BLI measurements show a pronounced reduction in binding for oligomerized 48 h-old P-Tau and P-TauΔK280. Tau showed less reduction in binding at 48 h. (C) Quantification of the binding (nm) of monomeric (0 h) and oligomeric (48 h) Tau versions at the end of the association phase (t = 150 s). Data are the mean + SD of the last five values of the association phase. Two-tailed Student’s t-test, unpaired, **** p < 0.0001.