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. 2022 Mar 22;23(7):3415. doi: 10.3390/ijms23073415

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Positive selection analysis of representative RLR genes. (a) Evolutionary relationships of MyeRLRs. The branches with dN/dS (ω) values > 1.0 are marked with red lines. (b) The expression level of five MyeRLRs in hemocytes. One-way ANOVA showed that the expression levels of MyeRLR2 and MyeRLR3 were significantly (p-value < 0.01, marked with **) more upregulated than those of other MyeRLRs. (c) Electrostatics of the RNA-binding surfaces of the MyeRLR RIG-I_C-RDs. The determination of RNA-binding sites refers to previous studies [49]. Positively charged surfaces are colored blue, and negatively charged surfaces are red. Dotted yellow line indicates RNA-binding loop.