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. 2021 Nov;100(5):513–525. doi: 10.1124/molpharm.121.000363

Fig. 4.

Fig. 4.

Cmpd-6 augments carvedilol-stimulated β2AR internalization. (A–C) Cell surface ELISA showing the cooperative effects of cmpd-6 on loss of cell-surface β2AR after a dose response of epinephrine (A), carvedilol (B), and ICI-118551 (C); normalized mean ± S.D, n = 5. (D) Bar graph showing mean difference in LogEC50 (versus DMSO control) for epinephrine (green, n = 5) and carvedilol (red, n = 5) in presence of cmpd-6. (E) Representative confocal images showing cmpd-6–mediated potentiation of lysosomal targeting of the β2AR upon stimulation with carvedilol (Carv). Scale bar on respective images is 10 μm. (F) Bar graph showing quantification of β2AR-YFP (green) colocalization with lysosomes (red); expressed as colocalization index ± S.D. as described in Materials and Methods. Statistical comparisons were made using one-way ANOVA with Bonferroni’s post hoc tests. **P < 0.01; ***P < 0.001.