Fig. 1.

Ectopic PKG2 is required for cGMP to inhibit the growth of LS174T colon cancer cells. Parental LS174T colon cancer cells and those engineered to express ectopic PKG2 were treated with 8Br-cGMP at the indicated concentrations. (A) The cGMP signaling was measured by immunoblotting for VASP phosphorylation at Ser239 (VASP-239P). The effect of 8Br-cGMP of parental and PKG2-expressing LS174T cells on (B) cell proliferation and (C) cell viability. Data show means, and error bars are S.D. ***P < 0.0001.