Fig. 2.

The inability of cGMP to affect proliferation is common among colon cancer cell lines. (A) Parental Caco2 and HCT116 colon cancer cells and those with inducible PKG2 expression were treated with 8Br-cGMP (100 µM) and doxycycline (Dox) to induce PKG2 expression as indicated. The cGMP signaling was measured by immunoblotting for VASP phosphorylation at Ser239 (VASP-239P). The effect of 8Br-cGMP treatment on (B) cell proliferation (hemocytometer), and (C) cell viability (trypan blue exclusion) is shown. Data show means, and error bars are S.D. *P < 0.05.