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Methodological design: (A) description of the protocol for exposure of pregnant rats to MeHg; (B) collection of biological material from the offspring (blood and spinal cord); (C) quantification of mercury (Hg) in the blood; (D) analysis of neuronal density by counting of the motor neuron cell bodies in hematoxylin and eosin-stained sections, and evaluation of MBP immunoreactivity using immunohistochemistry.
