Figure 2.

Detection of rod POS phagosomes depends on rhodopsin antibody usage. Freshly engulfed POS in early phagosomes before acidification retain both N-and C-terminal epitopes recognized by commercially available rhodopsin antibodies as indicated. Rhodopsin digestion proceeds in a step-wise fashion with early C-terminus cleavage resulting in loss of epitopes recognized by rhodopsin antibody 1D4 [67,70]. At late stages of phagolysosomal digestion, only LysoTracker labeling provides reliable phagosome labeling.