Figure 1.

Microfluidic chip design and conceptual use: (a) Schematic of the 5-channel (left) and 3-channel (right) microfluidic chip designs depicting the matrix, donor, recipient, and accessory channels. (b) Fully assembled chip loaded with blue ink to visualize the channels. (c) Schematic of the donor and recipient cells in the donor channel (DC) and recipient channel (RC). The matrix channel (MaC) can be filled with either Matrigel or PEGDA to permit size-based diffusion of either EVs and soluble factors or only soluble factors, respectively.