Figure 3: Impeded survival of other COMMD10-deficient tissue-resident macrophages.

(A) Gating strategy of large peritoneal macrophages (LPMs) defined as CD45⁺F4/80⁺CD11b⁺ ICAM2⁺. (B-G) LPMs or alveolar macrophages (AMs) from Commd10^fl/fl and LysM^ΔCommd10 mice were compared. (B) RT-PCR gene expression of Commd10 and Gata6 (n>5) (C) Fraction of LPMs among CD45⁺ cells. (D) LPM cell counts normalized to extracted volume (n>6). (E) Frequency of TIM4⁺, TIM4^lo and TIM4^- cells among LPMs (n>4). (F) Frequency of AMs (defined as CD45⁺F4/80⁺CD11C⁺SiglecF⁺) among CD45⁺ cells (n>3). (G) Mean fluorescence intensity (MFI) of CD11c expression by AMs (n=3). (H) Quantification of % chimerism in blood Ly6C^hi monocytes, LPMs and AMs assessed in mixed CD45.1 WT/CD45.2 LysM^ΔCommd10 BM chimeras at eight weeks post irradiation (n>9). (I-J) Frequency of (I) BrdU⁺ cells (n>7), and (J) Ki-67⁺ cells (n>3), among total LPMs (n>3). (K) Quantification of % chimerism in blood Ly6C^hi monocytes and lamina propria macrophages (lpMFs) as assessed in mixed CD45.1 WT/CD45.2 Cx3cr1^ΔCommd10 BM chimeras at eight weeks post irradiation (n=7). Data were analyzed by unpaired, two-tailed t-test and are presented as mean ± SEM with significance: *p < 0.05, **p < 0.01, ***p < 0.001. Experiments were repeated twice (A, C, E, H, I, K) or once ( B, D, F, G, H, J).