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. 2022 Mar 28;23(7):3694. doi: 10.3390/ijms23073694

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Western blot analysis of ACE2. The image illustrates (A) the glycosylated full-length ACE2 (120 KDa) and short-ACE2 isoforms (52 KDa) recognized with anti-ACE2-1 and (B) the glycosylated full-length ACE2 (120 KDa) detected by anti-ACE2-2. Antibodies were incubated on the same blot after membrane stripping and re-blotting. Blots were cut prior to hybridization. Each of the six lanes contains 20 µg of protein from semen of different donors. At least four independent experiments with different donors were performed. C+: protein from mouse testis.