Figure 2.

Representative schema of the ACE2 and short-ACE2 sequences and anti-ACE2 antibodies. Full-length ACE2 is composed of 805 amino acids (aas), while short-ACE2 is 459 aas length, thus sharing the sequence between the aas 347–805 (including the C-terminal domain). Numbers in blue correspond to the aas present in each sequence, while numbers in grey correspond to the sequence of aas missing in the short-ACE2 isoform. Three different antibodies were used: anti-ACE2-1 (abcam, ab15348) that recognizes the sequence between the aas 750–805; anti-ACE2-2 (Novus, NBP2-67692), recognizing the aas 200–230 from the full-ACE2; and anti-ACE2-3 (AF933, R&D Systems) recognizing the aas 18–640, mainly present in the full-ACE2 isoform.