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. 2022 Mar 28;23(7):3694. doi: 10.3390/ijms23073694

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Immunocytochemistry analysis of ACE2 on ejaculated human sperm cells. Panel (A) shows the location of the C-terminal domain of ACE2 recognized by anti-ACE2-1 (Abcam, ab15348), shared between both isoforms; Panel (B) shows the fluorescence pattern after immunocytochemistry analysis with anti-ACE2-2 (Novus, NBP2-67692), recognizing the full-length ACE2 only. DAPI was used to stain the nuclei. Top: two-laser image (anti-ACE2 antibody + DAPI); middle: sperm cells stained only with DAPI; bottom: sperm cells illustrating only ACE2 fluorescence patterns (scale bar = 5 µm). Negative controls for immunocytochemistry assays are shown in Supplementary Figure S1.